Detergent activation of the binding protein in the folate radioassay.

Clinical and laboratory observations on serum folate-binding protein.

We studied the effect of serum folate-binding protein (FBP) on folate radioassays and the relationship of the serum level of unsaturated FBP to the serum folate level in various clinical states. Our modification of a heat-extracted radioassay was compared to a whole serum radioassay. Our results confirmed the existence of elevated serum levels of unsaturated FBP in some normal subjects, in some...

Radioassay of serum folate.

We describe a radioassay procedure for serum folate, in which commercially available crystalline 3lactoglobulirt is used as the folate-binding agent. The folate binders present in serum are eliminated by heating serum in a boiling water bath for 15 mm to denature the proteins. Radioassayable serum folate is shown to be stable under these conditions, and the results for folate compare well with ...

Radiochemical method for measuring plasma clearance and urinary excretion of pteroylglutamic acid.

A radiochemical procedure is described for specific determination of pteroylglutamate in serum and urine. This method depends on denaturation of methyltetrahydrofolate with peroxide and measurement of the residual folate by a ligand-binding radioassay. The binding determinant for the radioassay is a folate-binding protein, partially purified from chronic myelogenous lekemia cells, that has low ...

Inadequate sample-preparation technique as a source of error in determination of erythrocyte folate by competitive binding radioassay.

Synthesizing and Characterizing Functionalized Short Multiwall Carbon Nanotubes with Folate, Magnetite and Polyethylene Glycol as Multitargeted Nanocarrier of Anti-cancer Drugs

Multifunctional nanomaterials showed graet advantages in drug delivery. Folic acid (FA) binding protein, a glycosyl phosphatidyl inositol anchored cell surface receptor for folate, is overexpressed in several human tumors, whereas it is highly restricted in normal tissues. Therefore, in this study, FA, polyethylene glycol (PEG), and Fe3O4 nanoparticles multifunctionalized short multiwall carbon...